Expanded Food Safety Investigation Act of 2025This bill provides that the Food and Drug Administration (FDA) may, under specified circumstances, request access to a concentrated animal-feeding operation (i.e., a stabled or confined animal-feeding operation of a specified size) to conduct microbial sampling.Specifically, the bill allows the FDA to request access if the FDA determines that sampling is necessary to facilitate an investigation of a foodborne-illness outbreak, determine the cause of an outbreak, or address other public health needs. Concentrated animal-feeding operations must provide reasonable access for sampling, including sampling of plants, animals, water, and the environment. The bill imposes penalties on operations that refuse to provide reasonable access. Data collected in sampling efforts under this bill must be shared with the Department of Agriculture and state and federal public health agencies to facilitate the detection, investigation, and prevention of foodborne illness.